A table summarizing the statistical analysis of community richness and composition. Paired t-tests were used to compare richness metrics between methods of trophic and plant parasite community detection. Generalized linear models were used to test the relationship between community richness and field of origin and sampled depth for morphology and mtMG based data. PERMANOVAs were used to test for significant explanation of variation in community Jaccard based dissimilarity based on field of origin and sampled depth for both morphology and mtMG based datasets.

SI Table 7: Community Richness and Composition Statistics,,,,,,,,,,,,,,,,
Paired T.Test,,,,,,,,,,,,,,,,
Annotated COX1 Trophic Richness vs. Morpho Trophic Richness,,,,,,,,,,,,,,,,
t-value,df,p-value,Significance ,Annoated COX1 Mean,Morpho Mean,,,,,,,,,,,
5.442,63,1.35E-06,***,2.0156,3.0625,,,,,,,,,,,
,,,,,,,,,,,,,,,,
Generalized Linear Models,,,,,,,,,,,,,,,,
Trophic Richness ~ Method + Field + Depth,,,,,,,,,,,,,,,,
,Estimate,Std. Error,t value,Pr(>|t|),Signficance,,,,,,,,,,,
(Intercept),3.56E+00,2.36E-01,15.109,2.00E-16,***,,,,,,,,,,,
Morphology Method,-1.93E-15,1.67E-01,0,1,,,,,,,,,,,,
Field C1,-1.25E-01,2.36E-01,-0.53,0.597006,,,,,,,,,,,,
Field O1,8.13E-01,2.36E-01,3.446,0.000785,***,,,,,,,,,,,
Field C2,-4.38E-01,2.36E-01,-1.855,0.065989,.,,,,,,,,,,,
25 – 50 cm,-7.50E-01,2.36E-01,-3.181,0.00187,**,,,,,,,,,,,
50 -75 cm,-7.50E-01,2.36E-01,-3.181,0.00187,**,,,,,,,,,,,
75-100 cm,-7.50E-01,2.36E-01,-3.181,0.00187,**,,,,,,,,,,,
,,,,,,,,,,,,,,,,
Post-hoc Annotated COX1 Trophic Richness ~ Field + Depth,,,,,,,Post-hoc Morpho Trophic Richness ~ Field + Depth,,,,,,,,,
,Estimate,Std. Error,t value,Pr(>|t|),Significance,,,Estimate,Std. Error,t value,Pr(>|t|),Significance,,,,
(Intercept),1.73E+00,3.21E-01,5.412,1.29E-06,***,,(Intercept),3.5625,0.32,11.132,6.39E-16,***,,,,
25 – 50 cm,-1.88E-01,3.43E-01,-0.547,0.586,,,25 – 50 cm,-0.75,0.3421,-2.192,0.033,*,,,,
50 -75 cm,-1.88E-01,3.43E-01,-0.547,0.586,,,50 -75 cm,-0.75,0.3421,-2.192,0.033,*,,,,
75-100 cm,9.38E-01,3.43E-01,2.737,0.008,**,,75-100 cm,-0.75,0.3421,-2.192,0.033,*,,,,
Field C1,3.75E-01,3.43E-01,1.095,0.278,,,Field C1,-0.125,0.3421,-0.365,0.716,,,,,
Field O1,8.50E-17,3.43E-01,0.000,1.000,,,Field O1,0.8125,0.3421,2.375,0.021,*,,,,
Field C2,1.88E-01,3.43E-01,0.547,0.586,,,Field C2,-0.4375,0.3421,-1.279,0.206,,,,,
,,,,,,,,,,,,,,,,
,,,,,,,,,,,,,,,,
,,,,,,,,,,,,,,,,
Paired T.Test,,,,,,,,,,,,,,,,
Annotated COX1 PP Richness vs. Morpho PP Richness,,,,,,,,,,,,,,,,
t-value,df,p-value,Significance ,Annoated COX1 Mean,Morpho Mean,,,,,,,,,,,
2.160,63,3.46E-02,*,0.5469,0.796875,,,,,,,,,,,
,,,,,,,,,,,,,,,,
Generalized Linear Models,,,,,,,,,,,,,,,,
Plant Parasite Richness ~ Method + Field + Depth,,,,,,,,,,,,,,,,
,Estimate,Std. Error,t value,Pr(>|t|),Signficance,,,,,,,,,,,
(Intercept),1.39E+00,2.25E-01,6.179,9.15E-09,***,,,,,,,,,,,
Morphology Method,-7.03E-01,1.59E-01,-4.418,2.19E-05,***,,,,,,,,,,,
Field C1,-1.56E-01,2.25E-01,-0.694,0.4889,,,,,,,,,,,,
Field O1,5.31E-01,2.25E-01,2.361,0.0199,*,,,,,,,,,,,
Field C2,2.19E-01,2.25E-01,0.972,0.333,,,,,,,,,,,,
25 – 50 cm,2.56E-16,2.25E-01,0,1,,,,,,,,,,,,
50 -75 cm,1.56E-16,2.25E-01,0,1,,,,,,,,,,,,
75-100 cm,-1.56E-01,2.25E-01,-0.694,0.4889,,,,,,,,,,,,
,,,,,,,,,,,,,,,,
Annotated COX1 PP Richness ~ Field + Depth,,,,,,,Morpho PP Richness ~ Field + Depth,,,,,,,,,
,Estimate,Std. Error,t value,Pr(>|t|),Significance,,,Estimate,Std. Error,t value,Pr(>|t|),Significance,,,,
(Intercept),0.4531,0.230,1.972,0.054,.,,(Intercept),1.08E+00,2.15E-01,5.009,5.61E-06,***,,,,
25 – 50 cm,-0.1875,0.246,-0.763,0.448,,,25 – 50 cm,-4.38E-01,2.30E-01,-1.901,0.062,.,,,,
50 -75 cm,0.2500,0.246,1.018,0.313,,,50 -75 cm,-3.13E-01,2.30E-01,-1.358,0.180,,,,,
75-100 cm,-0.1250,0.246,-0.509,0.613,,,75-100 cm,-5.63E-01,2.30E-01,-2.445,0.018,*,,,,
Field C1,0.1250,0.246,0.509,0.613,,,Field C1,4.49E-16,2.30E-01,0.000,1.000,,,,,
Field O1,0.2500,0.246,1.018,0.313,,,Field O1,5.00E-01,2.30E-01,2.173,0.034,*,,,,
Field C2,0.0625,0.246,0.254,0.800,,,Field C2,-3.13E-01,2.30E-01,-1.358,0.180,,,,,
,,,,,,,,,,,,,,,,
,,,,,,,,,,,,,,,,
Annotated COX1 Total Richness ~ Field + Depth,,,,,,,,,,,,,,,,
,Estimate,Std.Error,tvalue,Pr(>|t|),Significance,,,,,,,,,,,
(Intercept),2.58E+00,8.78E-01,2.936,0.005,***,,,,,,,,,,,
Field C1,1.38E+00,9.39E-01,1.465,0.149,,,,,,,,,,,,
Field O1,2.45E-15,9.39E-01,0.000,1.000,,,,,,,,,,,,
Field C2,4.06E+00,9.39E-01,4.327,6.16E-05,***,,,,,,,,,,,
25 – 50 cm,5.63E-01,9.39E-01,0.599,0.551,,,,,,,,,,,,
50 -75 cm,-9.38E-01,9.39E-01,-0.999,0.322,,,,,,,,,,,,
75-100 cm,-1.88E-01,9.39E-01,-0.200,0.842,,,,,,,,,,,,
,,,,,,,,,,,,,,,,
,,,,,,,,,,,,,,,,
,,,,,,,,,,,,,,,,
PERMANOVA,,,,,,,,,,,,,,,,
Trophic Jaccard Dist ~ Method*Field*Depth,,,,,,,,,,,,,,,,
,Df,SumsOfSqs,R2,F.Model,Pr(>F),Significance,,,,,,,,,,
Method,1,14.677,0.3466,82.0967,0.001,***,,,,,,,,,,
Field,3,1.609,0.03801,3.0009,0.004,**,,,,,,,,,,
Depth,3,1.55,0.03661,2.8904,0.002,**,,,,,,,,,,
Method*Field,3,1.739,0.04107,3.243,0.001,***,,,,,,,,,,
Method*Depth,3,1.694,0.04,3.1585,0.002,**,,,,,,,,,,
Field*Depth,9,2.339,0.05525,1.454,0.037,*,,,,,,,,,,
Method*Field*Depth,9,2.288,0.05404,1.4222,0.032,*,,,,,,,,,,
Residual,92,16.447,0.38841,,,,,,,,,,,,,
Total,123,42.345,1,,,,,,,,,,,,,
,,,,,,,,,,,,,,,,
Annotated COX1 Trophic Jaccard Dist ~ Field*Depth,,,,,,,,,Morpho Trophic Jaccard Dist ~ Field*Depth,,,,,,,
,Df,SumsOfSqs,MeanSqs,F.Model,R2,Pr(>F),Significance,,,Df,SumsOfSqs,MeanSqs,F.Model,R2,Pr(>F),Significance
Field,3,1.3990,0.4663,2.892,0.129,0.010,**,,Field,1,0.8424,0.8424,3.773,0.050,0.004,**
Depth,3,1.2591,0.4197,2.603,0.116,0.027,*,,Depth,3,1.9523,0.6508,2.915,0.116,0.001,***
Field:Depth,9,1.1200,0.1244,0.772,0.103,0.744,,,Field:Depth,3,1.4972,0.4991,2.235,0.089,0.008,**
Residuals,44,7.0949,0.1613,0.653,,,,,Residuals,56,12.5032,0.2233,0.744,,,
Total,59,10.8730,1.0000,,,,,,Total,63,16.7950,1.0000,,,,
,,,,,,,,,,,,,,,,
Plant Parasite Jaccard Dist ~ Method*Field*Depth,,,,,,,,,,,,,,,,
,Df,SumOfSqs,R2,F,Pr(>F),,,,,,,,,,,
Method,1,2.7865,0.09637,11.3233,0.001,***,,,,,,,,,,
Field,3,5.8765,0.20323,7.9598,0.001,***,,,,,,,,,,
Depth,3,1.0632,0.03677,1.4402,0.054,.,,,,,,,,,,
Method*Field,3,4.0315,0.13943,5.4607,0.001,***,,,,,,,,,,
Method*Depth,3,0.9596,0.03319,1.2998,0.101,,,,,,,,,,,
Field*Depth,9,2.44,0.08439,1.1017,0.244,,,,,,,,,,,
Method*Field*Depth,8,2.4062,0.08322,1.2222,0.079,.,,,,,,,,,,
Residual,38,9.3514,0.32341,,,,,,,,,,,,,
Total,68,28.9149,1,,,,,,,,,,,,,
,,,,,,,,,,,,,,,,
Annotated COX1 PP Jaccard Dist ~ Field*Depth,,,,,,,,,Morpho PP Jaccard Dist ~ Field*Depth,,,,,,,
,Df,SumsOfSqs,MeanSqs,F.Model,R2,Pr(>F),Significance,,,Df,SumsOfSqs,MeanSqs,F.Model,R2,Pr(>F),Significance
Field,3,5.5127,1.8376,6.825,0.495,0.001,***,,Field,1,1.8483,1.8483,5.844,0.123,0.001,***
Depth,3,0.5278,0.1760,0.654,0.047,0.860,,,Depth,3,1.5682,0.5228,1.653,0.105,0.031,*
Field:Depth,8,1.5974,0.1997,0.742,0.143,0.894,,,Field:Depth,3,1.1359,0.3786,1.197,0.076,0.235,
Residuals,13,3.5000,0.2692,0.314,,,,,Residuals,33,10.4380,0.3163,0.696,,,
Total,27,11.1379,1.0000,,,,,,Total,40,14.9904,1.0000,,,,
,,,,,,,,,,,,,,,,
,,,,,,,,,,,,,,,,
Annotated COX1 Total Jaccard Dist ~ Field*Depth,,,,,,,,,,,,,,,,
,Df,SumsOfSqs,MeanSqs,F.Model,R2,Pr(>F),Significance,,,,,,,,,
Field,3,4.0468,1.3489,3.442,0.161,0.001,***,,,,,,,,,
Depth,3,1.2885,0.4295,1.096,0.051,0.303,,,,,,,,,,
Field:Depth,9,2.5736,0.2860,0.730,0.102,0.996,,,,,,,,,,
Residuals,44,17.2455,0.3919,0.686,,,,,,,,,,,,
Total,59,25.1544,1.0000,,,,,,,,,,,,,